Genomics Laboratory (GL)
- real-time measurement of in vitro metabolic parameters using the Seahorse XFe96 S7800B instrument, available with the Cytation 5 reader, reporting oxygen consumption rate (OCR) or extracellular acidification rate (ECAR) by living cells in a 96-well format;
- scanning of membranes using the Odyssey XF (LICOR) scanner, facilitating near-infrared scans (e.g. detection of 2 proteins on a single Western blot membrane (PVDF, nitrocellulose) at the same time) or DNA visualization on gels;
- sorting cell suspensions into tubes and standard plates using the BD FACSMelody sorter (2 lasers, 6 colors), e.g. based on surface markers or live/dead dyes;
- preparation of libraries from cell/tissue preparations for single-cell RNASeq experiments using the Chromium iX instrument (10x Genomics);
- isolation of nucleic acids using the Maxwell RSC 48 instrument;
- analysis, detection and visualization of nanoparticles (e.g. “Nanoparticle Tracking Analysis”) using the NanoSight NS300 and the Micro-PLUS Kairos sorter together with the Optima XPN-80 ultracentrifuge;
- efficient transfection of various cell types using the Nucleofector 4D device and nucleofection technology.
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